ACLAR FILM EMBEDDING
Receive exclusive offers and updates from Oxford Academic. Abstract For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised. Transfer learning based deep CNN for segmentation and detection of mitoses in breast cancer histopathological images. For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised. Related articles in Web of Science Google Scholar. And finally, the film shows no signs of deterioration even after long term exposure to UV. But good results are indeed possible with the 2 mil product as well. Sign in via your Institution Sign in.
We can summarize some of the most desirable qualities of the ACLAR films, all of which are as true of the 2. Purchase Subscription prices and ordering Short-term Access To purchase short term access, please sign in to your Oxford Academic account above. For critical point drying, since the 7. Apparently, the more pliable nature of the thinner product makes it easier to get a good barrier seal against oxygen. Citing articles via Web of Science 3. For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised.
For a while, we believed that 2 mils is just not thick enough for applications in microscopy, and we started offering 7. And finally, the film shows no signs of deterioration even after long term exposure to UV.
Oxford University Press is a department of the University of Oxford. In general, we would recommend against use of this film when Embeddin is contemplated, unless of course there were no other alternatives which often times could be the case. It also serves as an excellent moisture barrier. But good results are indeed possible with the 2 mil product as well. Sign in via your Institution Sign in. Abstract For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised.
It furthers the University’s objective of excellence in research, scholarship, and education by publishing worldwide. One word of caution: Citing articles via Web of Science 3.
Microscopy in with a renewed editorial board. This article is also available for rental through DeepDyve. You do not currently have access to this article. Email alerts New issue alert. Resistance to osmium tetroxide: ACLAR 33C film can be cut embwdding a fairly ordinary scissors and reportedly, does not damage diamond knives during sectioning.
Related articles in Web of Science Google Scholar. We can summarize some of the most desirable qualities of the ACLAR films, all of which are as true of the 2. For critical point drying, since the 7. But now we have found that some users actually show a preference for the 2 mil thick product. Don’t have an account? This article was originally published embeddung.
Apparently, the more pliable nature of the thinner product makes it easier to get a good barrier seal against oxygen. Transfer learning based deep CNN for segmentation and detection of mitoses in breast cancer histopathological images. This method simultaneously offers transparency and thinness of the embedded blocks, absolute flatness of the sections, and easy removal of Epon from embedding molds.
Some comments specific to use in microscopy and histology laboratories The fluorinated-chlorinated polymers, because of the fluorinated aspect of the system, exhibit, in some respects almost PTFE-like properties, and therefore separates easily from all of the commonly used epoxy embedding formulations used in microscopy and histology.
But if you were tilm try the thinner product, in addition to finding it easier to seal, you will be happily surprised to find out that the thinner version of the product is also much less expensive!
ACLAR Film 33C, 7.8 mils thick, 10 x 8″”
The optical properties and UV transparency is essentially the emebdding. Hajime Sawada, Michiyo Esaki; A simple flat embedding method for the correlative light and electron microscopic immunocytochemistry, Journal of Electron MicroscopyVolume 47, Issue 5, 1 JanuaryPages —, https: Close mobile search navigation Aclsr navigation.
To purchase short term access, please sign in to your Oxford Academic account above. Because of the Cl content in the film, we would urge caution before using ACLAR film in conjunction with any EDS work because of the large count rate that would be coming from the presence of Cl in the film.
ACLAR®–Fluoropolymer Films
In the present technique tissue sections were attached to widely used silane-coated slides, processed, and embedded in Epon beneath non-adhesive transparent plastic films. Sign In Forgot password? That embefding why it is often times called an “embedding” film, which is why we call the 2 mil product “new and improved”. For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised.
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However, the manufacturer of ACLAR films has discontinued the production of the 5 mil film and only offers the film in 2 and 7. Sign In or Create an Account.

The Epon block can be easily removed from slides by heating. Most users should sign in with their email address. This is a polychlorotrifluoroethylene copolymer and this particular composition results in a film with very excellent optical properties, as well as good thermal and chemical stability.
Aclar Film Technical Data Sheet
You could not be signed in. Purchase Subscription prices and ordering Short-term Access To purchase short term access, please sign in to your Oxford Academic account above. Maximum dimension is 10″. Receive exclusive offers acar updates from Oxford Academic.
Don’t already have an Oxford Academic account? It can be applied to glass, such as a glass slide, without there being any real evidence for the presence of the film. A simple flat embedding method for the correlative light and electron microscopic immunocytochemistry Hajime Sawada.
Although the 5 mil film sectioned quite acceptably, we are told that the 2 mil product sections even easier.
